10yue13ri，yaboshoujibanzuowuyichuangailiangguojiazhongdianshiyanshilixingwangjiaoshouheliguoliangjiaoshouketizuzaizhiwuxueqikanmolecular plantzaixianfabiaoletiwei“unraveling the 3d genome architecture in plants: present and future”dezongshuwenzhang。
gaiwengaishulezhiwujiyinzudesanweizuzhijiegoutezheng，zhiwujiyinzuzaiyingduihuanjingbianhuashisanweijiegoudegaibian，yijibutongerbeitihesibeitizazhongzhongdengweijiyinsanweijiegoudechayi，jieshaoleransezhixiangguanrnacanyudedna-rnajiaohuzuoweijiyinzusanweijiegoudexindeyanjiucengmianzaizhiwujiyinzusanweijiegouzhongdezhongyaozuoyong，tantaole“ye-yexiangfenli”zaijiyinzusanweijiegoudexingchengzhongdeqianzaigongnengyiyi，zongjielemuqianhaiweizaizhiwuzhongyingyongdezuixinde“buyilaiyujiejinlianjie(proximity ligation-free)”dedna-dnajiaohubuhuojishuyijidna-rnajiaohubuhuojishu，zuihouduizhiwusanweijiyinzuxuedeyanjiufangxiangzuolezhanwang。
亚博手机版zhenheshengwumeitiaoransetizaixibaohezhongtongchanggezizhanjutedingdekongjian，chengweiransetijiangyu(chromosome territory, ct)。genjuransezhidehuoxinghezudanbaidexiushitezhengdeng，meitiaoransetikeyifenchenghuoyuederansezhiqushi(a compartment)heyizhixingransezhiqushi(b compartment)。genggaofenbianlvdesanweijiyinzutupuxianshi，zheixiea/bqushikeyijinyibufenweiyaqushi(subcompartment)，baokuohuoyueransezhizuchengdeyaqushi、duoshudanbaixiangguandeyizhixingdeyaqushi，heh3k9me2yudnajiajihuabiaojideyiransezhiyaqushideng。ransezhiqushibaohanyixieransezhijiaohujiegouyu(chromatin interacting domain)。zaihenduodongwuxibaozhong，qushineideransezhitongchangxingchenghenduobicixiangduijueyuan、neibuyouhenqiangjiaohudezijiaohujiegouyu(self-interacting domain)，chengweituopuxiangguanjiegouyu(topologically associating domain, tad)。dongwuransezhidetadjiegoushijiyinzhuanludiaokongdezhongyaojiegoudanyuan，tadexingchengtongchangshiyouctcf/cohesinfuhewujiedaode，taddebianjietongchangshizhuanluyinzictcfhecohesindanbaifuhewudejieheweidian。danshizhiwuzhongbingmeiyoufaxianctcfdanbai，henduozhiwuquecunzaitadleisijiegou(tad-like domain)。suiranyijingzaiyixiezhiwudetad-like domaindebianjiejiandingdaoyixiezhuanluyinzidemotifxulie，danshijiujingshishenmedanbaijiedaozhiwutadleijiegoudexingchengrengbuqingchu，yinci，jiandingctcf-likejueyuanzidanbai，tansuozhiwujiyinzhuanludiaokongderansezhisanweijiegoujichushiyigehenzhongyaodeyanjiufangxiang。ransezhihuan(chromatin loop)shijiyinzhuanludiaokongdejibendanyuan，shizhixianxingjulijiaoyuandelianggejiyinzuyuanjian（liruzengqiangzi，chenmozi，qidongzideng）zaidanbaizhihernadengdejiedaoxiaxingchengkongjianshangxianghukaojinderansezhijiegou。yishangsuoshudebutongcengjideransezhijiegou，zaihenduowuzhongzhongshibaoshoude，keyizaidabufenzhenheshengwuzhongjiandingdao。danshiduiyuyigewuzhongdebutongzuzhi、butongfayushiqihuobutongwaijietiaojianxia，ransezhidesanweijiegoucunzaihuoqianghuoruodedongtaibianhua，yushizheqiduijiyindeshikongchayibiaodajuyouqianzaidediaokongzuoyong。
figure 1. hierarchicalchromatin organization at multiple-length scales in plants.
亚博手机版guoqudesanweijiyinzuyanjiu，henduoshicongdna-dnajiaohu(dna-dna interactions)decengmianlaiyanjiuransezhidesanweijiegou。shijishang，rnazuoweixibaohedezhongyaozuchengchengfen，yuelaiyueduodeyanjiubiaomingqizairansezhijiegoudexingchengzhongbanyanzhongyaodejiaose。muqian，yidongwuxibaozuoweiyanjiuduixiang，yijingkaifalehenduoquanjiyinzushuipingjiancedna-rnajiaohudejishu(lirumargi，grid-seq，char-seqdeng)，zheixieyanjiujieshileransezhixiangguanrnadeyixiezuoyongmoshi，biaomingqiduiransezhijubugouxiangjuyoudiaokongzuoyong。suiranmuqianzaizhiwuyanjiuzhongyeyouyixieguanyurnayingxiangransezhijubujiegoudebaodao，danshizheixieyanjiudoushizhenduitedingweidiankaizhan。weilaideyanjiuxuyaozaizhiwuzhongjianliquanjiyinzushuipingdedna-rnajiaohubuhuojishu，daguimojiandingransezhixiangguanderna，bingyanjiuzheixiernaduijiyinzusanweijiegoudeyingxiangyijizaizhiwushengzhangfayuguochengzhongfahuidezuoyong。
ye-yexiangfenli(liquid-liquid phase separation, llps)shizhizaiguobaoherongyezhonghuixingchengliangzhongbutongnongdudekeyiwendinggongcundeyexiangdexianxiang。zaixibaozhong，mouxiedanbaizhihehesuandengshengwudafenzikeyifashengxianghuzuoyongningjizaiyiqi，tongguollpsdejizhixingchengwumodeyedishenzhishiwumoxibaoqi。zuixindeyanjiufaxian，llpskeyiqudongyixieransezhiqushihuadexingcheng，biaomingllpszaijiyinzusanweijiegoudexingchengzhongkenengfahuizhongyaozuoyong。zaizhiwuzhong，yijingyouhenduoguanyullpsxiangguandewumoningjuti(condensate)debaodao，henduowumoningjutiyuzhiwudeshengzhangfayudiaokongyijijiyinzujiegouxiangguan。suiranllpsjiujingruhejiangjiyinzusanweijiegou、jiyinzhuanludiaokong、zhiwushengzhangfayudengshengwuxueguochenglianxiqilaihaibuqingchu，zheijiangshiyigefeichangyouyiyideyanjiufangxiang。
muqianzhiwusanweijiyinzuyanjiudejieguohenduodoushijiyu“proximity-ligation”deyuanlikaifadejishuhuodede。yinweizheixiejishushijiyudnamoduanpeiduilianjiedeyuanli，yincizhinengjiancedaojiandandedna-dnajiaohu，erduiyufuzade、julihenyuandedna-dnajiaohuyijidna-rnajiaohu，zexuyaokaifaxindebuyilaijiejinlianjiede(ligation-free)jishuhedna-rnajiaohubuhuojishu，huozhekeyitongshibuhuoduozhongjiaohudemulti-waydefangfa。ciwai，xuyaokaifadanxibaoshuipingsanweijiyinzuyanjiujishuyijiduozuxue(multimodal omics)jishu，congdanxibaoshuipingyanjiujiyinzusanweijiegouduijiyinzhuanludediaokongzuoyong。
亚博手机版yaboshoujibanshengkeyuanboshishengouyangweizhihehexinxixueyuanbiyeboshishengxiongdanboshiweigailunwendegongtongdiyizuozhe，shengkeyuanlixingwangjiaoshouhexinxixueyuanliguoliangjiaoshouweigailunwendegongtongtongxunzuozhe。xiangguangongzuodedaoguojiazirankexuejijin、guojiazhongdianyanfajihuahezuowuyichuangailiangguojiazhongdianshiyanshikaifangketidezizhu。
